Respiration and Oxidative Phosphorylation in Treponema pallidum

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Respiration and oxidative phosphorylation in Treponema pallidum.

Exogenous and endogenously generated reduced pyridine nucleotides caused marked stimulation of O(2) uptake when added to treponemal cell-free extracts, which indicated that terminal electron transport was coupled to the consumption of O(2). Oxidation of reduced nicotinamide adenine dinucleotide (NADH) was shown to correlate stoichiometrically with O(2) reduction, suggesting that NADH was being ...

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Shape of Treponema pallidum.

Treponema pallidum was found to be not helical, but a flat wave twisted into one to five different planes per cell.

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Treponema pallidum in nerve fibres.

Ultrathin sections of a rabbit scrotal syphiloma were examined by electron microscopy. Treponemes were observed in the endo-, peri-, and epineurium of the nerve fibre. The significance of these findings, in that infection may be transmitted via the nerve fibres and pain reduced by damage to the afferent fibres, are discussed.

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Mucopolysaccharidase of Treponema pallidum.

Treponema pallidum (Nichols strain) exhibited mucopolysaccharidase activity. Acidic mucopolysaccharides were broken down more rapidly by viable treponemes than by heat-inactivated treponemes or membrane filtrates of treponemal suspensions. Ouchterlony immunodiffusion demonstrated the occurrence of antibodies to the hyaluronidase-like enzyme within syphilitic sera. After intratesticular inoculat...

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Phagocytosis and avirulent Treponema pallidum.

SIR-Uptake of avirulent T. pallidum (Nichols strain) was demonstrated in an in vitro system. Motile treponemes were harvested after culture for 7 days in spirolate broth (Izzat, Knox, and Wende, 1971). Non-motile treponemes were also obtained by keeping some of these at 4°C. for 18 hours. Macrophages were obtained from the peritoneal cavity of a rabbit bywashing with 100 ml. Hanks's balanced sa...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1978

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.21.2.462-473.1978